Author: katiehenry created date: 5/27/2014 8:20:10 pm. If your ecoli exhibit both these properties at once, it is highly likely it is due to transformation and not just simple mutation page 41 ap bio pglo lab help i need help with this lab. Pglo transformation lab prep for lab quiz learn with flashcards, games, and more for free. Transformation of e coli with green fluorescent protein (gfp) cat #223/ap08 in this experiment, students will explore the biological process of bacterial transformation using e coli and plasmid dna at the end of the activity.
Free essay: bacterial transformation lab report backround: the plasmid pglo contains an antibiotic-resistance gene, ampr, and the gfp gene is regulated by. In the pglo bacterial transformation kit, students transform bacteria with a gene from a bioluminescent jellyfish to express a new protein, green fluorescent protein (gfp) they can turn the genes on and off to study gene regulation. 1 bacterial transformation with pglo overview transformation = modification of a bacterium by the uptake and incorporation of exogenous dna determine the transformation efficiency of the competent. Activity 4: transformation of e coli using green fluorescent protein information for teachers transformation of cells is a widely used and versatile tool in genetic engineering and is of critical importance in the development of molecular biology.
Quizlet provides pglo lab activities, flashcards and games start learning today for free. Free essay: connor lauffenburger 3/17/13 pglo transformation lab report i introduction the purpose of this experiment was to show the genetic transformation. Bacterial transformation plasmid for regulated expression of gfp. Transformation lab report introduction transformation is the transfers of virulence from one cell to another, through the transferring of genetic material.
Student manual pglo transformation lesson 1 introduction to transformation in this lab you will perform a procedure known as a genetic transformation. Blake's honors biology site blake's honors in our procedure, we created both a +pglo solution and a -pglo solution introduction- to explain the pglo transformation lab, we must first go all the way back to dna structure. Labbench activity key concepts i: bacterial transformation genetic transformation occurs when a host organism takes in foreign dna and expresses the foreign gene. Some bacteria have another method of transferring dna and producing recombinants that does not require conjugation the conversion of one genotype into another by the introduction of exogenous dna (that is, bits of dna from an external source) is termed transformation transformation was. Pglo plasmids are how is the pglo plasmid introduced into the e coli cell the general process by which foreign dna is introduced into a cell is called transformation there are several ways to transform dna into an e coli cell.
Best answer: first you have to understand what genetic transformation is genetic transformation literally means change caused by genes and it involves inserting genes into an organism in order to change their phenotype transformation can also occur in nature when a bacterial cell.
In this lab experiment, my lab partners and i examined the process by which bacteria takes in foreign genetic material and incorporates it into its own dna, also known as bacterial transformation we learned that a heat shock caused the dna in the bacteria to be partially denatured. The ampicillin resistance gene (carried on the recombinant pglo plasmid we are using) produces a protein -lactamase, which disables the ampicillin molecule -lactamase cleaves a lab 16 - bacterial transformation. You will be using a plasmid called pglo (p = plasmid) because the gene being carried on is the code for the fluorescent protein there is another important gene on this pglo plasmid---an antibiotic resistant gene that bacterial transformation author. Computer 6a advanced biology with vernier 6a - 1 pglo bacterial transformation introduction to transformation in this lab, you will perform a procedure known as genetic transformation. A student researched experiment about a pglo lab in this lab mutagenesis was preformed by adding a transposon to the pglo plasmid.
Wwwwasatchedu. Bacterial transformation using the pglo plasmid safety notes: sterile techniques should always be used when working with live bacterial cultures. Title: pglo transformation lab introduction: genetic transformation is a change caused by genes, involving the insertion of a gene into an organism to change the organism s trait.